differentiation 68 cd68  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: Luteolin in Safflower Leaves Suppresses Microglial Inflammation Through FOXO3-Mediated Trem2 Transcription

doi: 10.3390/antiox14121495

Figure Lengend Snippet: Safflower leaves inhibited microglial activation in APP/PS1 mice. ( A ) Ionized calcium-binding adaptor molecule 1 (Iba1) immunohistochemistry (IHC) in the brain. The arrow points to a microglial process. ( B ) Statistical analysis of Iba1 positive microglia area, n = 3 per group. ( C ) Cluster of differentiation 68 (CD68) IHC in the brain. ( D ) Statistical analysis of CD68 integrated optical density, n = 3 per group. ( E ) Expression of microglial inflammatory genes in a public Alzheimer’s disease (AD) dataset. Data was retrieved directly from the AlzData database (visualization of GEO dataset GSE26972 ), n = 3 per group. ( F ) Iba1 and triggering receptor expressed on myeloid cells 2 (TREM2) colocalization. ( G ) Relative abundance of Iba1 + /TREM2 + cells in the brain, n = 3 per group. ( H ) TREM2 immunofluorescence (IF) in the brain. ( I , J ) Statistical analysis of TREM2 integrated optical density in the hippocampus ( I ) and the cortex ( J ), n = 3 per group. Values represent the mean ± SEM. The results were analyzed by one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The cluster of differentiation 68 (CD68) antibody (Abclonal, Wuhan, China), diluted 1:100 in PBS containing 1% BSA, were used to stain activated microglia.

Techniques: Activation Assay, Binding Assay, Immunohistochemistry, Expressing, Immunofluorescence

Journal: Antioxidants

Article Title: Luteolin in Safflower Leaves Suppresses Microglial Inflammation Through FOXO3-Mediated Trem2 Transcription

doi: 10.3390/antiox14121495

Figure Lengend Snippet: Luteolin inhibited microglial inflammatory responses and increased triggering receptor expressed on myeloid cells 2 (TREM2) in lipopolysaccharides (LPS)-activated N9 cells. ( A ) Cell viability, n = 6 per group. ( B – F ) mRNA levels of tumor necrosis factor-α ( Tnfα ), interleukin-1β ( Il1β ), interleukin-6 ( Il6 ), nitric oxide synthase 2 ( Nos2 ) and cyclooxygenase-2 ( Cox2 ), n = 3 per group. ( G ) Calcein AM staining. ( H , I ) Statistical analysis of cell area ( H ) and cell branches number ( I ), n = 3 per group. ( J ) Cluster of differentiation 68 (CD68) immunofluorescence (IF). ( K ) Statistical analysis of CD68 integrated density, n = 3 per group. ( L ) Representative images of western blot results for TREM2, p-spleen tyrosine kinase (SYK), SYK, p-extracellular signal-regulated kinase (ERK), ERK, toll-like receptor 4 (TLR4) and β-ACTIN. ( M – P ) The results were quantified and normalized to β-ACTIN, n = 4 per group. Values represent the mean ± SEM. The results were analyzed by one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The cluster of differentiation 68 (CD68) antibody (Abclonal, Wuhan, China), diluted 1:100 in PBS containing 1% BSA, were used to stain activated microglia.

Techniques: Staining, Immunofluorescence, Western Blot

Journal: Nature Communications

Article Title: Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging

doi: 10.1038/s41467-025-65297-8

Figure Lengend Snippet: a , b UMAP embedding of 1180 macrophage nuclei from sciatic nerves color-coded by subtype ( a ) or age ( b ). c Dot plot highlighting the marker genes used to identify the macrophage subtypes from A. Marker genes can be found in Table . d Relative percentage of Mϕs subtypes across ages. Steady state epi- and endoneurial Mϕ, circulating-derived phagocytic Mϕ, and chronic-inflammation Mϕ were more prevalent in sciatic nerves from 2-3, 15-16, and 20-30 months old mice, respectively. e Dot plot showing the expression levels of the marker genes used to identify the macrophage clusters, at the different time points. Markers for resident steady state Mϕ ( Cx3cr1, Mrc1, Csf1r ) are more expressed at 2-3 months old, those for circulating-derived phagocytic Mϕ (Cd74, Hspa5, Cd44, C1qa, Grn, Cd68), are more expressed at 15-16 months old, and those belonging to chronic inflammation ( Tgfβr1, Kynu) are more expressed in the 20-30 months old samples. f Representative immunofluorescence images of sciatic nerve samples show increased CD45 + /MRC1 + Mϕs in nerves from 2-3 months old mice, CD68 + /GRN + Mϕs in nerves from 15-16 and 20-30 months old mice, and CD45 + /TGF βR1+ in nerves from 20-30 months old mice. Yellow or pink arrowheads point to CD45 + /MRC1+ or CD68 + /GRN+ or CD45 + / TGF βR1 + cells. g Quantification of the number of CD45+ cells relative to the total number of cells. n = 7 - 9 - 10, biological replicates. h Quantification of the number of CD45 + /MRC1 + cells relative to the total number of CD45 + cells. n = 4 - 4 - 5 biological replicates. i Quantification of the number of CD68 + / GRN+ cells relative to the total number of CD68+ cells. n = 6 - 6 - 5. j Quantification of the number of CD45 + /TGF βR1 + cells relative to the total number of CD45 + cells. n = 3 - 5 - 5, biological replicates. Data are presented as mean values +/− SD. Source data for panels is provided as a Source Data file.

Article Snippet: Cluster of differentiation 68 (CD68) 1:250 , Mouse , MCA1957 Biorad Hercules, CA, USA.

Techniques: Marker, Derivative Assay, Expressing, Immunofluorescence